产品货号：
GS2096
中文名称：
HspAI内切酶
英文名称：
HspAI
产品规格：
500U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：HspAI内切酶，HspAI限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | G↑CGC |
| 来源 | Isolated from an E.coli strain that carries the cloned HspA I gene from Haemophilus species A1 |
| Assayed on | Lambda DNA |
| Optimal buffer | Y?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);50 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 20-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Blocked by CG methylation 5-G(5mC)GC-3/3-CG(5mC)G-5.|Not blocked by methylation 5-GCG(5mC)-3/3-CGCG-5.|Cut hemimethylated site:5- G(5mC)GC-3/3-CGCG-5. |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | AspLEI?^,BstHHI^,CfoI^,GlaI^,HhaI^,R9529,HinP1I |
相关搜索：HspAI内切酶，HspAI限制性内切酶